Impressum

Email

fon +49 (0)7531 88-4808

room L1051

Education

since 10/2014

University of Konstanz, Germany

PhD student

 

09/2012-07/2013

National Taiwan University, Taiwan

Research Assistant

 

09/2010-06/2012

National Taiwan University, Taiwan

Master Studies in Chemistry

 

09/2006-06/2010

National Cheng Kung University, Taiwan

Bachelor of Science, Department of Chemistry

 

 

Research Interest

The astonishing material properties of biominerals are due to hierarchical structures and complex morphologies, which are tailor made for the specific biological functions. However, the detailed mechanisms of how organisms precisely regulate mineralization remain a longstanding enigma. It is believed that macromolecules are responsible for directing the nucleation and growth of calcium carbonate.1,2 The major objective of my research is an advanced understanding of the protein-mineral interactions during the early stages of calcium carbonate precipitation, and how they influence and direct the course of crystalline. Our approach is to express the proteins found in sea urchin3,4 or mollusk shell5,6 recombinantly and to investigate these proteins either individually or combinatorially, in order to study the protein-mineral interaction as well as synergistic protein-protein interactions. We will use various in situ and ex situ techniques, including crystallization assay7, cryo-TEM, isothermal titration calorimetry, and solid-state NMR, to gain information on the size, stability, chemical composition, structure, and binding energy of protein-mineral complexes.

 

(1) Falini, G.; Albeck, S.; Weiner, S.; Addadi, L. Science 1996, 271, 67.

(2) Suzuki, M.; Saruwatari, K.; Kogure, T.; Yamamoto, Y.; Nishimura, T.; Kato, T.; Nagasawa, H. Science 2009, 325, 1388.

(3) Mann, K.; Poustka, A. J.; Mann, M. Proteome Sci. 2008, 6.

(4) Mann, K.; Wilt, F. H.; Poustka, A. J. Proteome Sci. 2010, 8.

(5) Michenfelder, M.; Fu, G.; Lawrence, C.; Weaver, J. C.; Wustman, B. A.; Taranto, L.; Evans, J. S.; Morsel, D. E. Biopolymers 2003, 70, 522.

(6) Zhang, C.; Zhang, R. Q. Mar. Biotechnol. 2006, 8, 572.

(7) Gebauer, D.; Völkel, A.; Cölfen, H. Science 2008, 322, 1819.

 

 

Publications

1. Y.-C. Huang, Y. Mou, T. W.-T. Tsai, Y.-J. Wu, H.-K. Lee, S.-J. Huang, J. C. C. Chan*, Calcium-43 NMR Studies of Polymorphic Transition of Calcite to Aragonite, J. Phys. Chem. B 116, 14295 (2012).

 

2. Y.-C. Huang, H.-H. Chang, Y. Mou, P. Chi, S.-C. Luo, J. C. C. Chan*, Purification of recombinant nacre-associated mineralization protein AP7 fused with MBP, Protein Expression and Purification 100, 26 (2014).

 

3. M. Pendola, G. Jain, Y.-C. Huang, D. Gebauer, J. S. Evans*, A nacre protein forms mesoscale hydrogels that “hijack” the biomineralization process within a seawater environment, CrystEngComm 18, 7675 (2016).

 

4. G. Jain, M. Pendola, Y.-C. Huang, D. Gebauer, J. S. Evans*, A model sea urchin spicule matrix protein, rSpSM50, is a hydrogelator that modifies and organizes the mineralization process, Biochemistry 56, 2663 (2017).

 

5. G. Jain, M. Pendola, Y.-C. Huang, D. Gebauer, J. Juan-Colás, S. Johnson, J. S. Evans*, Functional prioritization and hydrogel regulation phenomena created by a combinatorial pearl-associated 2-protein biomineralization model system, Biochemistry 56, 3607 (2017).

Yu-Chieh Huang (PhD Student)